The police are investigating a fatal incident that occurred in Eildon, just before midnight on Sunday.
Investigators were told that a group of individuals had a physical altercation in front of a licensed establishment on Goulburn valley Highway.
One of the males was believed to have been hit before he fell, and then hit his head against the ground.
The paramedics tried to treat him but he died on the spot.
The police are now speaking to several witnesses in order to determine the exact circumstances of the man’s demise.
Anyone with information or who may have witnessed the incident is urged to call Crime Stoppers on 1800 333 000 or submit a confidential crime report at www.crimestoppersvic.com.au.
